BRAINCAKEChromatography of Amino Acids
After a protein has been hydrolysed, the mixture of amino acids can be separated and identified by chromatography, usually paper chromatography or thin-layer chromatography (TLC).
A pencil baseline is drawn near the bottom of the paper or plate (ink would dissolve and run). Small spots of the hydrolysed mixture, and of known amino acids for comparison, are placed on the line. The paper is stood in a covered tank of solvent with the solvent level below the baseline. As the solvent rises, each amino acid moves a different distance, depending on its relative affinity for the stationary and mobile phases. When the solvent is near the top, the paper is removed and the solvent front is marked.
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All 12 lessons in Amines, Amides, Amino Acids and Proteins · All Edexcel A-level Chemistry topics