DNA can only be synthesised in the 5' to 3' direction.This means that the DNA polymerase can only add nucleotides to the 3'OH group. So the strand can only grow in one direction.An RNA polymerase synthesises an RNA primer on the DNA template.Then the DNA polymerase add nucleotides to the primer.However, the two strands run in opposite directions. DNA helicase runs along the DNA unwinding it so DNA polymerase is following the replication fork.So DNA polymerase can synthesise DNA continuously on the leading strand but has to synthesise DNA discontinuously in the opposite direction on the lagging strand.This means that DNA is synthesised in sections called Okazaki fragments on the lagging strand.A short RNA primer is laid down on the lagging strand.Small sections of DNA are synthesised using the RNA primers.The RNA primers are then removed and the sections filled in with DNA nucleotides.The Okazaki fragments are then joined by DNA ligase to form a continuous strand.
DNA Replication. 
Continuous and Dis-continuous Replication
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Specification: OCR Biology A (H020), AS-level, 2.1.3 Nucleotides and nucleic acids. Topic: Biological Molecules.
Key terms in this lesson
- replication fork
- The Y-shaped region where the DNA double helix is unwound by DNA helicase, exposing the two strands for replication.
More in Biological Molecules
- RNA
- DNA Extraction
- Role of RNA
- DNA Replication
- Replication Errors
- The Genetic Code
- Protein Synthesis
- The role of tRNA, mRNA and rRNA
All 63 lessons in Biological Molecules · All OCR AS-level Biology topics