1. The sample is ground with a pestle and mortar to break down the cell walls and leave only the cell membrane.2. Detergent is then added to break down the cell membrane and release the contents of the cell.3. Salt is added to break the bonds between the DNA and the histone proteins associated with it.4. Protease enzyme is added to digest the histones that are associated with the DNA.5. Alcohol causes DNA to precipitate out of the solution between the layer of sample and layer of alcohol and can removed using a glass rod.