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Thin Layer Chromatography

OCR AS-level BiologyBiological MoleculesLesson 35 of 63

Thin layer chromatography is used to separate components of a mixture.

It can also be used as a analytical technique to identify the components of the mixture.

It consists on a stationary phase which is a thin layer of silica on a glass or other inert solid surface with one end standing in a jar of organic solvent called the mobile phase. The solvent is which is about 1cm deep.

Diagram

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