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Measuring Rate of Reaction

Edexcel AS-level BiologyEnzymesLesson 6 of 14

The rate of an enzyme-controlled reaction is the change in the amount of substrate or product per unit time. It can be measured by following either:
• how quickly a product appears, for example collecting the oxygen released when catalase breaks down hydrogen peroxide, using a gas syringe or an upturned measuring cylinder
• how quickly a substrate disappears, for example the loss of starch when amylase acts, tested with iodine solution (blue-black to orange-brown), or measured with a colorimeter

Diagram

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Key terms in this lesson

colorimeter
An instrument that measures the amount of light of a particular colour absorbed by, or transmitted through, a solution.

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