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Transgenic Plants

Edexcel A-level BiologyGene TechnologyLesson 8 of 17

A transgenic plant contains a gene from a different species. The most common method of producing one uses the soil bacterium Agrobacterium tumefaciens.

Method
1. In nature, Agrobacterium infects wounded plants and transfers a section of its Ti plasmid, called T-DNA, into the plant's chromosomes, causing a tumour (crown gall).
2. Scientists remove the tumour-causing genes from the T-DNA and insert the desired gene, together with a marker gene such as herbicide or antibiotic resistance.
3. The modified bacteria are mixed with small pieces of plant tissue, such as leaf discs. Only the T-DNA is transferred into the plant cells' chromosomes.

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