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Cloning Plants

Edexcel A-level BiologyGene TechnologyLesson 9 of 17

Plants can be cloned to produce many offspring that are genetically identical to the parent. This is useful for multiplying a valuable or genetically modified plant quickly.

Micropropagation (tissue culture)
1. A small piece of tissue, called an explant, is cut from the parent plant, often from a meristem such as a shoot tip.
2. The explant is sterilised, for example with dilute bleach, to kill microorganisms on its surface.
3. It is placed on a sterile agar medium containing glucose, amino acids, minerals and plant hormones (auxins and cytokinins).
4. The cells divide by mitosis to form an undifferentiated mass called a callus.

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Key terms in this lesson

aseptic technique
Working methods that prevent microorganisms from contaminating cultures or equipment.

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