BRAINCAKEGene Sequencing
Revision notes for Gene Sequencing in Edexcel Biology B (9BI0). Every lesson in BrainCake comes with R.E.C.I.P.E. recall steps, a quick check and spaced reviews.
- The GenomeThe genome is all of the genetic material in an organism.
- The Polymerase Chain ReactionThe polymerase chain reaction (PCR) is a technique used to make millions of copies of a specific section of DNA in a short time.
- How PCR WorksEach cycle of PCR has three stages, controlled by the thermal cycler.
- DNA SequencingDNA sequencing means working out the order of bases (A, T, C and G) in a piece of DNA.
- Using DNA SequencingOnce DNA can be sequenced quickly and cheaply, the sequences can be compared and analysed using computers.
- Using DNA Sequencing to Manage DiseaseDNA sequencing is now an important tool for preventing, diagnosing and treating disease.
- Introns and Satellite DNAMost of the genome does not code for proteins.
- DNA ProfilesA DNA profile is a pattern, usually shown as a series of bands or peaks, that represents the number of repeats a person has at several satellite DNA…
- Gel ElectrophoresisGel electrophoresis separates DNA fragments according to their length.
- Southern Blotting and Gene ProbesAfter gel electrophoresis the DNA bands cannot be seen, and the soft gel is hard to handle.
- Forensic ScienceForensic scientists use DNA profiling to link people to crime scenes, to rule suspects out and to identify bodies.
- Paternity TestingDNA profiling can show whether a man is the biological father of a child.
On the specification
- 7.1 Using gene sequencing