BRAINCAKEGene Sequencing
Revision notes for Gene Sequencing in Edexcel Biology (9BI0). Read the start of any lesson here, then sign in free for the whole lesson, the R.E.C.I.P.E. recall steps and the quiz.
- The GenomeThe genome is all of the genetic material in an organism.
- The Polymerase Chain ReactionThe polymerase chain reaction (PCR) is a technique used to make millions of copies of a specific section of DNA in a short time.
- How PCR WorksEach cycle of PCR has three stages, controlled by the thermal cycler.
- DNA SequencingDNA sequencing means working out the order of bases (A, T, C and G) in a piece of DNA.
- Using DNA SequencingOnce DNA can be sequenced quickly and cheaply, the sequences can be compared and analysed using computers.
- Using DNA Sequencing to Manage DiseaseDNA sequencing is now an important tool for preventing, diagnosing and treating disease.
- Introns and Satellite DNAMost of the genome does not code for proteins.
- DNA ProfilesA DNA profile is a pattern, usually shown as a series of bands or peaks, that represents the number of repeats a person has at several satellite DNA…
- Gel ElectrophoresisGel electrophoresis separates DNA fragments according to their length.
- Southern Blotting and Gene ProbesAfter gel electrophoresis the DNA bands cannot be seen, and the soft gel is hard to handle.
- Forensic ScienceForensic scientists use DNA profiling to link people to crime scenes, to rule suspects out and to identify bodies.
- Paternity TestingDNA profiling can show whether a man is the biological father of a child.