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Substrate Concentration

OCR AS-level BiologyEnzymesLesson 13 of 20

At low substrate concentration not all the active sites of the enzymes are filled as there are relatively few molecules of substrate.

If the substrate concentration is increased, the rate of reaction increases as the substrate concentration is limiting.

More active sites are filled and more enzyme-substrate complexes are formed so more product is formed per unit time.

Diagram

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Key terms in this lesson

active site
The region of an enzyme, with a shape complementary to its substrate, where the substrate binds and the reaction is catalysed.
enzyme-substrate complex
The temporary complex formed when a substrate binds to the active site of an enzyme.

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