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Test for Starch

The test for starch uses iodine dissolved in potassium iodide solution, which is orange-brown.A few drops of the iodine solution are added to the sample. Solid samples, such as a slice of potato, can be tested directly, or the sample can be ground up with water first.If starch is present the colour changes from orange-brown to blue-black. If there is no starch the solution stays orange-brown.The blue-black colour forms because iodine (as triiodide ions) fits inside the helical coils of amylose, forming a coloured complex.The test is qualitative: it shows whether starch is present but not how much. It can be used to follow the digestion of starch by amylase, because the blue-black colour no longer appears once all the starch has been hydrolysed to maltose.

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Specification: OCR Biology A (H020), AS-level, 2.1.2 Biological molecules. Topic: Biological Molecules.

Key terms in this lesson

amylose
The unbranched, helical component of starch, made of alpha-glucose monomers joined by glycosidic bonds, that forms a blue-black complex with iodine.

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