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Evidence for Active Transport

Edexcel AS-level BiologyCell Transport MechanismsLesson 17 of 18

Several lines of evidence show that some substances enter cells by active transport rather than by diffusion.

1. Movement against a concentration gradient. Some cells build up ions at a much higher concentration than in their surroundings. Diffusion can only move a substance down its gradient, so continued uptake when the inside concentration is already higher means the substance is moving against the gradient. For example, if a root contains 50 mmol dm−3 of potassium ions while the solution around it has 5 mmol dm−3, the inside concentration is 10 times the outside.

2. Dependence on respiration. Active transport needs ATP from respiration. Uptake falls sharply or stops when:

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Key terms in this lesson

active transport
The movement of molecules or ions across a membrane against their concentration gradient, using energy from ATP.
concentration gradient
The difference in the concentration of a substance between two regions, which determines the direction of net diffusion.

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