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Translation

Once the mRNA leaves the nucleus a ribosome attaches to the start codon (triplet of bases) which is always AUG.A tRNA molecule with the complementary anticodon UAC bound to the amino acid methionine attaches to the mRNA. Complementary base pairing and hydrogen bonding occurs between the codon of the mRNA and anticodon of the tRNA.Another tRNA whose anticodon is complementary to the next codon binds to the mRNA by base-pairing. Each tRNA molecule is attached to a specific amino acid.The large sub unit of the ribosome acts as an enzyme peptidyl transferase and uses energy from the hydrolysis of ATP to join the two amino acids together with a peptide bond.The ribosome moves along the mRNA strand as the first tRNA molecule no longer bound to its amino acid detaches from the mRNA. Another amino acid bound tRNA molecule with an anticodon complementary to the next codon binds to the mRNA at the next codon and the process of joining amino acids together and the ribosome moving along the mRNA to the next codon continues so that about 15 amino acids are joined every second.The peptide chain grows until a stop codon is reached. The ribosome detaches.Up to 50 ribosomes may be bound to one mRNA chain at a time moving along the mRNA behind each other.

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Specification: AQA Biology (7401), AS-level, 3.4.2 DNA and protein synthesis. Topic: DNA, Genes and Protein Synthesis.

Key terms in this lesson

hydrogen bond
A weak electrostatic attraction between a slightly positive hydrogen atom on one polar molecule and a slightly negative atom, such as oxygen or nitrogen, on another.
hydrolysis
The breaking of a covalent bond within a molecule using a water molecule.
complementary base pairing
The specific pairing of bases by hydrogen bonds, adenine with thymine (or uracil) and guanine with cytosine.
ATP
Adenosine triphosphate, a nucleotide that acts as the immediate energy source in cells, releasing energy when hydrolysed to ADP and phosphate.
ribosome
A small organelle, made of RNA and protein, that is the site of protein synthesis.

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