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Substrate Level Phosphorylation

AQA A-level BiologyRespirationLesson 5 of 12

Substrate-level phosphorylation is the formation of ATP without the involvement of the electron transport chain. A phosphate group is added to each molecule of triose phosphate using inorganic phosphate from the cytoplasm. This forms two triose bisphosphate molecules. After a series of steps ATP is generated from the removal of the phosphate groups from each of the triose bisphosphate molecules. NAD is reduced by the hydrogen atoms released.

Diagram

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Key terms in this lesson

glycolysis
The first stage of respiration, occurring in the cytoplasm, in which glucose is split into two molecules of pyruvate with a net yield of 2 ATP and reduced NAD.
pyruvate
The three-carbon end product of glycolysis, which enters the mitochondrion for the link reaction in the presence of oxygen or is reduced to lactate or ethanol in its absence.
electron transport chain
A series of protein complexes in the inner mitochondrial (or thylakoid) membrane that pass electrons along a chain of redox reactions, pumping protons across the membrane to generate a proton gradient.
ATP
Adenosine triphosphate, the universal energy currency of cells, formed by adding an inorganic phosphate to ADP and hydrolysed to release energy for cellular reactions.

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