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Thin-Layer Chromatography

OCR A-level Organic ChemistryChromatography and Qualitative AnalysisLesson 2 of 10

Thin-layer chromatography (TLC) is a quick way to find how many components a mixture contains, and to compare them with known substances.

The stationary phase is a thin layer of silica (SiO2) or alumina (Al2O3) on a glass, metal or plastic plate. The mobile phase is a solvent. Separation is by adsorption.

Diagram

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Key terms in this lesson

stationary phase
The phase in chromatography that does not move, and interacts with the components of a mixture to different extents.
mobile phase
The phase in chromatography, a liquid solvent or gas, that moves through or over the stationary phase carrying the mixture's components.

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