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Gas Chromatography

OCR A-level Organic ChemistryChromatography and Qualitative AnalysisLesson 4 of 10

Gas chromatography (GC) separates volatile components of a mixture, and is sensitive enough to detect very small amounts.

• The mobile phase is an unreactive carrier gas, such as helium or nitrogen.
• The stationary phase is a high-boiling liquid coated on an inert solid support (or on the inside wall) inside a long, thin, coiled capillary column.
• The column is kept inside an oven so the sample stays as a gas.

A small sample is injected, vaporises and is carried through the column by the carrier gas. Components separate by their relative solubility in the liquid stationary phase: a component that is more soluble in the liquid spends longer dissolved in it and moves through the column more slowly.

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Key terms in this lesson

stationary phase
The phase in chromatography that does not move, and interacts with the components of a mixture to different extents.
mobile phase
The phase in chromatography, a liquid solvent or gas, that moves through or over the stationary phase carrying the mixture's components.

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