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Ultracentrifugation

A high-speed centrifuge is able to separate out small particles by subjecting them to a centrifugal force.This can be used to isolate cell organelles or analytically to determining the sizes of some particles or the molecular weights of large molecules.The centrifuge spins the filtered homogenate at a very high speed.At each speed the heaviest organelles are pushed to the bottom and form a pellet.The rest of the organelles are suspended in the fluid above called the supernatant. The supernatant can then be spun again at a higher speed.The order organelles are isolated are:

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Specification: AQA Biology (7401), AS-level, 3.2.1.3 Methods of studying cells. Topic: Cell Structure.

Key terms in this lesson

supernatant
The fluid remaining above a pellet after centrifugation, containing the lighter organelles not yet sedimented.

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