BRAINCAKEThe ELISA Test
ELISA stands for enzyme linked immunosorbant assay. Antibodies are used to detect the amount of protein in a sample. It is extremely sensitive and can detect small amounts of the protein.
The antigen is placed on a slide. Excess antigen is washed off.
The antibody for the antigen to be tested is added to the slide. The antibody binds to the antigen and the excess is washed off.
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Key terms in this lesson
- pathogen
- A microorganism, such as a virus, bacterium, fungus or protoctist, that causes disease.
- antigen
- A protein or glycoprotein, usually on a cell surface, that is recognised as foreign by the immune system and triggers an immune response.
- antibody
- A Y-shaped protein, made of four polypeptide chains with variable regions complementary to a specific antigen, produced by B plasma cells.
More in Cell Recognition and the Immune System
- Herd Immunity
- Vaccination Programme Failure
- Ethics
- MMR Vaccine Case Study
- Structure of HIV Virus
- Replication of HIV Virus
- AIDS
- Antibiotics
All 30 lessons in Cell Recognition and the Immune System · All AQA AS-level Biology topics