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Measuring RQ with a Respirometer

OCR A-level BiologyRespirationLesson 19 of 19

A respirometer measures the rate of oxygen uptake by small organisms such as germinating seeds or woodlice. It can also be used to find the RQ.

Diagram

The organisms are placed in a tube on a gauze platform above soda lime (or potassium hydroxide solution), which absorbs carbon dioxide. A control tube contains glass beads of the same volume as the organisms. Both tubes are connected by a U-tube manometer containing coloured fluid, and are kept in a water bath at constant temperature, because gas volume changes with temperature and pressure. The control tube compensates for these changes.

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Key terms in this lesson

respiratory quotient
The ratio of the volume of carbon dioxide produced to the volume of oxygen used in respiration over the same time.

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