Measuring RQ with a Respirometer
A respirometer measures the rate of oxygen uptake by small organisms such as germinating seeds or woodlice. It can also be used to find the RQ.
The organisms are placed in a tube on a gauze platform above soda lime (or potassium hydroxide solution), which absorbs carbon dioxide. A control tube contains glass beads of the same volume as the organisms. Both tubes are connected by a U-tube manometer containing coloured fluid, and are kept in a water bath at constant temperature, because gas volume changes with temperature and pressure. The control tube compensates for these changes.
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Key terms in this lesson
- respiratory quotient
- The ratio of the volume of carbon dioxide produced to the volume of oxygen used in respiration over the same time.
More in Respiration
- Anaerobic Respiration - Plants and Microorganisms.
- Anaerobic Respiration - Mammals
- Respiratory Substrates
- Energy Values of Carbohydrates, Lipids and Proteins
- Respiration of Lipids
- Respiration of Proteins
- Respiratory Quotient
- Calculating RQ
All 19 lessons in Respiration · All OCR A-level Biology topics