BRAINCAKEImmobilised Enzymes
An immobilised enzyme is an enzyme that is attached to, or held inside, an insoluble material so that it cannot mix freely with the substrate solution. The substrate solution flows over or through the enzyme, and the product flows out without the enzyme.
Methods of immobilisation
• Adsorption: enzyme molecules are bound to the surface of an insoluble support such as clay, porous carbon or glass beads, by hydrophobic interactions and ionic bonds. Enzymes may leak off.
• Covalent bonding: enzymes are covalently bonded to a support such as cellulose or collagen fibres. They are held very firmly, but the bonding can alter the active site.
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More in Cloning and Biotechnology
- Culturing Microorganisms
- Aseptic Technique
- Serial Dilution
- Standard Growth Curve
- Batch Fermentation
- Continuous Fermentation
- Controlling Conditions in Fermenters
- Uses of Immobilised Enzymes
All 21 lessons in Cloning and Biotechnology · All OCR A-level Biology topics