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Chi-Squared Test- Dihybrid Crosses

Edexcel A-level BiologyGenetic InformationLesson 21 of 30

For a dihybrid cross there are usually four phenotype categories, so there are 4 − 1 = 3 degrees of freedom, and the critical value at p = 0.05 is 7.82.

Worked example: two Drosophila heterozygous for two genes on different chromosomes are crossed. The expected ratio is 9 : 3 : 3 : 1. The 160 offspring are 94 wild type, 26 with vestigial wings only, 32 with ebony body only and 8 with both.

Null hypothesis: there is no significant difference between the observed numbers and those expected from a 9 : 3 : 3 : 1 ratio.

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Key terms in this lesson

degrees of freedom
The number of categories in a statistical test minus one.
critical value
The value of a test statistic, such as chi-squared, that must be reached or exceeded for a difference to be significant.
null hypothesis
A statement that there is no significant difference between observed and expected results, and any difference is due to chance.

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