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Temperature as a Limiting Factor

AQA A-level BiologyEnergy for Biological ProcessesLesson 12 of 17

Temperature affects photosynthesis mainly through enzymes. The light-independent reaction (Calvin cycle) is catalysed by enzymes such as rubisco, so it is the stage most affected by temperature. The light-dependent reaction is less affected, because absorbing light is a physical process.

At low temperatures, molecules have little kinetic energy, so there are few successful collisions between enzymes and substrates and few enzyme-substrate complexes form. The rate of photosynthesis is low. As the temperature rises, the rate increases up to an optimum.

Above the optimum the rate falls, for two reasons:

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Key terms in this lesson

rubisco
The enzyme that catalyses the fixation of carbon dioxide onto ribulose bisphosphate (RuBP) in the Calvin cycle; it is the rate-limiting enzyme of photosynthesis.

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