Skip to content

Rate calculations for enzyme activity

The rate of a reaction tells us how quickly it happens. For an enzyme, we can measure how much product forms in a given time, or how much substrate is used up. Rate = amount of product formed ÷ time. The units depend on what is measured, such as cm3/s for a gas or g/s for a mass. For example, 24 cm3 of gas in 40 s gives 24 ÷ 40 = 0.6 cm3/s.

Sometimes we only measure the time a reaction takes to finish. Then rate = 1 ÷ time, so a reaction that finishes in 20 s has a rate of 1 ÷ 20 = 0.05 per second. The shorter the time, the faster the rate.

On a graph of product against time, the gradient of the line is the rate. A steeper line means a higher rate. The gradient is the change in product divided by the change in time. A reaction slows down as the substrate is used up, so the line becomes flat when the reaction has finished.

Read the text

Read the text and highlight anything you think is important. When you go on, the text is hidden and you answer from memory.