BRAINCAKERetention Times
In gas chromatography (GC) and high performance liquid chromatography (HPLC), the components of a mixture are carried through a column by the mobile phase and separated by how strongly they interact with the stationary phase.
The retention time is the time taken for a component to pass through the column: from the moment the sample is injected to the moment that component reaches the detector (measured at the peak maximum). A component that is more soluble in, or more strongly adsorbed onto, the stationary phase has a longer retention time.
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Key terms in this lesson
- chromatogram
- The output of a chromatography technique, showing a peak for each separated component, used to identify and measure the components of a mixture.
More in Modern Analytical Techniques
- Chromatography
- Thin-Layer Chromatography
- High Performance Liquid Chromatography
- Gas Chromatography
- Nuclear Magnetic Resonance
- Chemical Shift
- 13C NMR Spectroscopy
- 1H NMR Spectroscopy
All 15 lessons in Modern Analytical Techniques · All Edexcel A-level Chemistry topics