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Super Resolved Fluorescence Microscopy

OCR AS-level BiologyComponents of Living SystemsLesson 20 of 21

Super resolved fluorescence microscopy can achieve a resolution of 0.2μm using visible light.

There are two different types. One combines many very small images to produce a larger image and the other superimposes many images with normal resolution to produce a high resolution image.

The first type uses the technique of stimulated emission depletion.

Diagram

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Key terms in this lesson

resolution
The ability to distinguish two objects that are close together as separate entities, which determines how much detail can be seen.

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