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Light Microscope

Edexcel AS-level BiologyEukaryotic CellsLesson 2 of 30

The light microscope (optical microscope) uses visible light focused by glass lenses. Light passes up through a thin specimen on a slide, then through the objective lens and the eyepiece lens. The total magnification is the product of the two:

total magnification = objective × eyepiece

so a ×40 objective with a ×10 eyepiece gives ×400.

Diagram

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Key terms in this lesson

resolution
The smallest distance between two points that can still be seen as two separate points; higher resolution shows more detail.

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