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Effect of Substrate Concentration

AQA AS-level BiologyEnzymesLesson 10 of 17

At low substrate concentration not all the active sites of the enzymes are filled as there are relatively few molecules of substrate.

If the substrate concentration is increased, the rate of reaction increases as the substrate concentration is limiting.

More active sites are filled and more enzyme-substrate complexes are formed so more product is formed per unit time.

Diagram

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Key terms in this lesson

active site
The region of an enzyme, formed by the tertiary structure, with a shape complementary to its substrate, where the substrate binds.
enzyme-substrate complex
The temporary structure formed when a substrate binds to the active site of an enzyme.

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