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Gram Stain

OCR AS-level BiologyComponents of Living SystemsLesson 5 of 21

All bacterial cell walls are made of peptidoglycan. Before staining bacteria are often colourless.

Gram positive bacteria have a thick net-like layer of peptidoglycan containing a substance called teichoic acid.

When crystal violet followed by iodine is applied, the crystal violet-iodine complex is trapped within the cell wall and is not washed away when the sample is dehydrated with alcohol.

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