Mycobacterium spp. are bacteria associated with TB and other infections. A sample from a person is placed on a glass slide, and carbolfuchsin in a lipid solvent is applied.The slides are then heated and then washed with a solution of dilute acid and alcohol.Mycobacterium spp. retain the stain and appear red under the microscope.If methylene blue is applied, bacteria that do not retain the carbolfuchsin are stained blue.
Acid-Fast Technique
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Specification: OCR Biology A (H020), AS-level, 2.1.1 Cell structure. Topic: Components of Living Systems.
More in Components of Living Systems
- The Light Microscope
- Preparing Samples
- Staining
- Gram Stain
- Magnification
- Resolution
- The Eyepiece Graticule
- Electron Microscopy
All 21 lessons in Components of Living Systems · All OCR AS-level Biology topics